Application of virucidal preparations
As the first commercial laboratory in Poland, we have implemented a test of virucidal effectiveness of preparations intended for disinfection. The tested and proven efficacy of disinfectants against viruses is of particular importance in the medical field. Patients and staff are faced with a multitude of pathogens that cause serious diseases on a daily basis.
Another area where virucides cannot be lacking is veterinary medicine.
Livestock buildings are an ideal environment for the development and transmission of diseases between animals. Livestock diseases are one of the key causes of farmers’ losses. Therefore, disinfection and prevention play a key role in this area.
Virucidal preparations during an epidemic
During the SARS-CoV-2 epidemic, the virucidal effect of chemical disinfectants has become very important in the areas of everyday life as well.
Biocides appeared in every home, industrial plants, shops and shopping malls. The effectiveness of this type of preparations is therefore a very important element in preventing a pandemic. Only agents whose operation has been confirmed by appropriate tests may be registered in the Office for Registration of Medicinal Products. Thus admitted to trading.
Virucidal effectiveness test
Testing the virucidal effectiveness of chemical disinfectants is a process that requires a specialized laboratory and qualified personnel.
We evaluate the effectiveness against viruses on strains identified as model in particular methodologies. The research process itself is very time-consuming and complicated. For testing, we use specially cultivated and maintained cell lines, and the tests are carried out on living human cells, e.g. lung epithelial cells. We infect these cells with viruses during the test. As a result of the infection, the cells die. The study also takes into account the cytotoxicity of a given preparation.
If the preparation turns out to be cytotoxic, i.e. in addition to the pathogen, it also kills cells, its biocidal effectiveness becomes impossible to define. This is usually the case with very strong preparations containing at least a few active substances.
An interesting fact is that in this respect products based on hypochlorous acid turn out to be very delicate. The process of testing virucidal effectiveness ends with statistical calculations and preparation of a report based on the guidelines of a given methodology.
We perform virucidal effectiveness tests for producers for the purposes of registration with the Biocidal Products Registration Office. We also provide the service of registration of biocides.
We test virucidal effectiveness according to the following standards
PN-EN 14476+A2:2019-08 Chemical disinfectants and antiseptics. Quantitative suspension method to determine virucidal activity in the medical field – Test method and requirements (Phase 2 / Phase 1)
The PN-EN 14476 + A2: 2019-08 methodology is applicable to products for hygienic hand disinfection by rubbing, hygienic hand washing, disinfecting tools by immersion, disinfecting surfaces by wiping and disinfecting textiles.
The model strains in the full spectrum of activity are the viruses: Poliovirus type 1, Murine norovirus and Adenovirus type 5. On the restricted spectrum: Murine norovirus and Adenovirus type 5, and on enveloped viruses: Vaccinia virus (ATCC® VR-1549 ™). In addition, we offer the possibility of conducting tests on a strain of human coronavirus with the designation: (HCoV-229E).
PN-EN 16777:2019-01 Chemical disinfectants and antiseptics. Quantitative surface method to determine the virucidal activity of chemical disinfectants used in the medical field on non-porous surfaces, without mechanical action. Test method and requirements (phase 2 / step 2)
Research acc. PN-EN 16777 methods relate to the assessment of the disinfection efficiency of non-porous surfaces, including the surface of medical devices, without mechanical action.
The test strains are: Murine norovirus, Adenovirus type 5 and Vaccinia virus, Polio virus cannot be tested with this methodology. Products for disinfection with a full virucidal spectrum must also be tested according to methodology PN-EN 14476 + A2: 2019-08.
PN-EN 17111:2018-12 Chemical disinfectants and antiseptics. Quantitative Carrier-based Method for Determining Virucidal Activity for Instruments Used in the Medical Field – Test Method and Requirements (Phase 2, Step 2)
The PN-EN 17111: 2018-12 standard is used in testing products for disinfecting tools, and the model strains < are Murine norovirus and Adenovirus type 5, in a limited spectrum (> parvovirus and its effectiveness against enveloped viruses are tested against the strain Vaccinia virus.
PN-EN 14675 :2015 Chemical disinfectants and antiseptics – Quantitative suspension test for the determination of the virucidal activity of chemical disinfectants and antiseptics used in the veterinary field – Test method and requirements (Phase 2, Step 1)
This standard refers to products used in the field of veterinary medicine, nutrition, breeding, production, transport and sale of all animals, excluding slaughter as the initial stage of food processing and introduction to the processing industry.
The test strain for the veterinary standard is Bovine enterovirus Type 1.
PN-EN 17122:2020-04 Chemical disinfectants and antiseptics – Quantitative surface method to determine the virucidal activity of chemical disinfectants and antiseptics used in the veterinary field on non-porous surfaces – Test method and requirements – Phase 2, Step 2
This document specifies the test method and minimum requirements for the virucidal activity of chemical disinfectants that are uniform, physically stable, when diluted with hard water or, for ready-to-use products, with water. This document refers to products used in the veterinary field for disinfection, without mechanical action, of non-porous surfaces, i.e. in feeding, breeding, production, veterinary facilities, transport and disposal of all animals, excluding slaughter as the initial phase of food processing and introduction. to the processing industry.
Using this document, it is possible to determine the virucidal activity of undiluted products.
Porcine Parvovirus is used in this document because the Bovine Enterovirus E virus (formerly Bovine Enterovirus Type 1 (ECBO)) used in the EN 14675 suspension test cannot be used for the surface test due to titer loss on drying. Porcine Parvovirus has comparable resistance to the ECBO virus.
DIN EN 17430 Chemical disinfectants and antiseptics – Hygienic handrub virucidal – Test method and requirements (phase 2 / step 2); German and English version prEN 17430: 2019
For the offer of testing the virucidal effectiveness of preparations for disinfection, please contact the Customer Service Office, tel: +48 22 379 25 20 or email@example.comRegistration of Biocidal Products